Composite

Part:BBa_K5131006:Design

Designed by: Yu Han   Group: iGEM24_Squirrel-Shanghai   (2024-09-30)


pGEX-GST-nsp5-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1726
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 770
    Illegal XhoI site found at 1721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1165
    Illegal SapI.rc site found at 167


Design Notes

NA


Source

The sequence of nsp5 is from SARS-Cov-2 Wuhan-hu-1.

References